Overview The Höpfler lab is looking for a postdoctoral researcher to lead a project in the field of RNA biology and gene regulation with a strong interest in driving development of sequencing methods to identify mRNA decay substrates and decipher degradation mechanisms.The successful candidate will join the Dynamics of Protein Synthesis and RNA Decay Group. Their work will address how cells dynamically control the stability of messenger RNAs by selective degradation during protein synthesis by the ribosome. The lab studies this problem in the context of both fundamental molecular mechanisms and disease-related regulation. The project is embedded in the ERC-funded DECODE-PMD project to better understand peptide-mediated mRNA decay (PMD).The role offers the opportunity to join a young, ambitious and curiosity-driven lab at the CRG, one of Europe's prime institutes for molecular life sciences, in a dynamic Barcelona research environment.Key responsibilities Development of sequencing approaches to identify PMD substratesBioinformatic data analysis and visualizationIdentification and mechanistic characterization of PMD candidate substratesPresentation of data at international conferences and manuscript preparationLeading and managing international collaborationsActive participation in lab meetings and contribution to a culture of technical excellence and scientific curiosityAbout the Höpfler lab How do cells accurately tune protein production according to cellular needs? This question is linked to human health, as aberrant protein levels are commonly associated with diseases such as developmental defects, cancer, or neurodegenerative diseases where aggregation-prone proteins may accumulate in neurons.In the "Dynamics of protein synthesis & RNA decay" lab we investigate how cells tune protein production by adjusting the stability of messenger RNAs (mRNAs). Each cell expresses around 12,000 different mRNAs at any given time. To control this transcriptome—and thus protein synthesis—cells adjust half-lives for individual mRNAs from minutes to several days. We study peptide-mediated mRNA decay (PMD), where the nascent protein is recognized to trigger degradation of the encoding mRNA during translation by the ribosome, as recently demonstrated for tubulin mRNAs.Using an interdisciplinary approach spanning biochemistry, structural biology, cell biology, genetics, and advanced sequencing methods, we aim to answer:What are the factors and molecular mechanisms driving PMD?How are these mechanisms controlled, and what are the consequences of impaired mRNA turnover?What is the transcriptome-wide scope of PMD regulation?Insights into mRNA decay mechanisms have relevance to human biology and disease and may inform the development of optimized mRNA-based therapeutics like mRNA vaccines.Recent publications and additional information can be found on the lab website: https://www.Crg.Eu/en/programmes-groups/hopfler-lab Contact: Markus Höpfler markus.hopfler@crg.euwho we would like to hire We are particularly interested in candidates with a hybrid wet lab + bioinformatics background with a track record in RNA biology or gene regulation, but we will consider applications with related expertise. Experience with sequencing approaches to study RNA turnover or with massively parallel reporter assays ( MPRAs ) will be beneficial.Must Have Bioinformatics experience in the analysis of (RNA) sequencing dataExperience with common lab techniques in cell & molecular biologyThe candidate must hold a PhD degree, preferably in cell/molecular biology or bioinformatics, or related fields (or expect to finish the degree before starting)A strong publication record commensurate with career stageAbility to pursue research projects independentlyDesirable but not required Experience with massively parallel reporter assays of any kindExperience with studying mRNA turnover on a transcriptome-wide scaleBackground in RNA biology, translational control or related fieldsLanguages Fluency in written and spoken EnglishSkilled with data analyses software (R/Python)Experience with using high performance computing clustersCompetences Curiosity and critical thinkingRigor, organization and autonomyStrong analytical, interpersonal, and communication skillsThe Offer – Working Conditions Contract duration: Technical and scientific activities contract (estimated duration 2 years)Estimated annual gross salary: Salary will be commensurate with qualifications and consistent with our pay scalesTarget start date: from October 2026 (flexible)We provide a stimulating environment with state-of-the-art infrastructures and professional career development opportunities.We offer and promote a diverse and inclusive environment and welcome applicants regardless of age, disability, gender, nationality, ethnicity, religion, sexual orientation or gender identity.The CRG is committed to reconciling a work and family life for its employees and offers extended vacation periods and flexible working hours.Application and Selection Application requirements:A complete CV including contact details and publications.Contact details of two to three referees.All applications must be addressed to Dr. Markus Höpfler and submitted online on the CRG Career site - http://www.Crg.Eu/en/content/careers/job-opportunitiesSelection process:Pre-selection based on qualifications and expertise reflected in the candidate CVs;
merit-based.Interview with Hiring Manager and possibly a selection panel.Offer letter: Successful candidate will receive a job offer with start date, salary and working conditions.Deadline: Please submit your application before 31/05/2025.
#J-18808-Ljbffr